Immobilization of thiol peptides and proteins on poly(thioglycoloyloxyethyl methacrylate) gel
نویسنده
چکیده
PoIy(thioglycoloyloxyethyl methacrylate) gel has been prepared. The reactivity of its SH groups was checked with [C]phenyl isothiocyanate. Immobilization of thiol peptides and proteins to a carrier can be performed after its activation with 6,6'-dithiodinicotinic acid. The attached compounds can be released with 50 mmol dm dithiothreitol or can be hydrolyzed with enzymes or acids without decomposition of the carrier.
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